Role of CRISPR/Cas9 in Agriculture

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CRISPR/Cas9 is one of the most popular tools used in the world of science today. But what is so special and unique about this technology, and why is it the most favored topic of researchers?

The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 system is the most recent gene-editing technique, allowing geneticists and medical researchers to edit portions of the genome by deleting, inserting, or modifying DNA sequences. It is composed of two main elements. The first component is the Cas9 protein, an endonuclease that is capable of double-stranded cleavage of DNA.

The other component is single-guide RNA molecules, which form a complex with the Cas9 protein and direct it to its genomic site of action by complementary base pairing with the target region. A naturally existing genome editing mechanism in bacteria was used to develop this technique. CRISPR/Cas9 systems start as a part of the bacterial innate immune system, where they serve as a memory platform for the host’s immune response to fight viral infection successfully.

The process behind it:

Bacteria capture snippets of DNA from invading viruses and use them to create DNA segments known as CRISPR arrays. These arrays enable bacteria to remember viruses (or closely related ones). If the viruses strike again, the bacteria employ the CRISPR arrays to generate RNA segments that target the viruses’ DNA. The bacteria then use Cas9 or another comparable enzyme to break the DNA apart, rendering the virus inoperable.

CRISPR

For genome editing by CRISPR/Cas9, researchers create a small piece of RNA with a short ‘guide’ sequence that attaches to a specific target sequence of DNA in a genome. The RNA also binds to the Cas9 enzyme. Like in bacteria, the modified RNA is used to recognize the DNA sequence, and the Cas9 enzyme cuts the DNA at the targeted location. After cutting the DNA, researchers utilize the cell’s DNA repair mechanism to add or remove genetic material fragments or alter the DNA by replacing an existing segment with a tailored DNA sequence.

CRISPR/Cas9 has invaded the scientific industry with a storm because of being cheaper, more accurate, and more efficient than other genome editing methods. It has a role to play in several fields like drug discovery, biomedical research, prevention of diseases, etc. Furthermore, CRISPR/Cas9 has played an essential role in agriculture by making it feasible to modify crops to make them more nutritious, tasty, disease-resistant, and drought-resistant. It has been employed in an increasing number of monocot and dicot plant species to improve yield, quality, and nutritional value and introduce or improve resistance to biotic and abiotic stressors.

Role of CRISPR- Cas9:

A fundamental part of the genome editing process is identifying target genes that determine phenotypes of interest, such as susceptibility to viruses, other pathogens, resistance to herbicides, or adverse environmental factors. Assessment of natural variation or systematic genome-wide screens is also a powerful approach to identify target genes.

Cas9
Illustration of CRISPR-Cas9

In potatoes, CRISPR-Cas9 was used to knock out the gene encoding granule-bound starch synthase (GBSS) in one round of transfection, resulting in potato plants that produce amylopectin starch a highly desirable commercial trait. The CRISPR-Cas9 technology was utilized in cucumber to inactivate the eukaryotic translation initiation factor gene elF4E. The resultant non-transgenic homozygotic mutant plants were resistant to the potyviruses Zucchini yellow mosaic virus and Papaya ringspot mosaic virus, and Cucumber vein yellowing virus (Genus Ipomovirus). Engineering genetic resistance to viruses and other pathogens offers enormous promise for managing illnesses that have no natural resistance, such as maize fatal necrosis disease and tomato brown rugose fruit virus.

In the future, practical photorespiration efficiency improvements in crops like maize, rice, and wheat can significantly boost food production for the world’s rising population while using the same cultivated areas and destroying additional forest areas for agricultural purposes. Producing crops with higher food quality through genome editing will also contribute to food security.

CONCLUSION:

CRISPR/Cas9 โ€“ a unique prokaryotic, viral defense system โ€“ has undoubtedly become one of the most influential and versatile platforms for engineering biology today and has indeed signified the importance of scientific research. Although biosafety concerns remain, genome editing is a promising technology to contribute to food production to benefit the growing human population.

~by Gauri Chincholkar

CLICK HERE to learn more about how CRISPR is being utilized in reverse aging technologies.

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